Two Electrode Clamp for Oocytes

Details

• Sophisticated and extremely fast two-electrode voltage and current clamp (V/C) amplifier with PI-controller for recordings from oocytes
• Push buttons for comfortable and fast selection of MODE of OPERATION
• Differential potential registration and high-voltage current source output, automated electrode resistance test mode which can be used even with the electrodes impaled in an oocyte
• VC OUTPUT LIMITER, COMMAND FILTER, INTEGRATOR and SERIES RESISTANCE COMPENSATION for fine tuning of VC circuit
• Current transient compensation prevents data acquisition system from clipping
• Digital DISPLAYS for current, voltage and electrode resistance
• Four-pole BESSEL filter for current
• No virtual ground needed for recording membrane currents
• OSCILLATION SHUT-OFF unit prevents cells from damage
• Standard current range is ±150 µA into 1 MOhm. Current headstages with selectable current ranges (x0.1, x1, x2, x5 or x0.1, x0.2, x0.5, x1) are also available
• Easy operation with all major data acquisition systems, remote selection of MODE of OPERATION, telegraphing (monitoring) outputs for current sensitivity and filter.

Science

Disse, P., Aymanns, I., Ritter, N., Peischard, S., Korn, L., Wiendl, H., Pawlowski, M., Kovac, S., Meuth, S. G., Budde, T., Strutz-Seebohm, N., Wünsch, B., & Seebohm, G. (2023). A novel NMDA receptor test model based on hiPSC-derived neural cells. Biological Chemistry. https://doi.org/10.1515/hsz-2022-0216

Tribiños, F., Cuevas, P., Cornejo, I., Sepúlveda, F. V., & Cid, L. P. (2023). A new family of glutamate-gated chloride channels in parasitic sea louse Caligus rogercresseyi: A subunit refractory to activation by ivermectin is dominant in heteromeric assemblies. PLOS Pathogens, 19(3), e1011188. https://doi.org/10.1371/journal.ppat.1011188

Peischard, S., Möller, M., Disse, P., Ho, H. T., Verkerk, A. O., Strutz-Seebohm, N., Budde, T., Meuth, S. G., Schweizer, P. A., Morris, S., Mücher, L., Eisner, V., Thomas, D., Klingel, K., Busch, K., & Seebohm, G. (2022). Virus-induced inhibition of cardiac pacemaker channel HCN4 triggers bradycardia in human-induced stem cell system. Cellular and Molecular Life Sciences, 79(8). https://doi.org/10.1007/S00018-022-04435-7

Bechthold, E., Schreiber, J. A., Ritter, N., Schepmann, D., Daniliuc, C., Seebohm, G., & Wünsch, B. (2022). Synthesis and biological evaluation of conformationally restricted GluN2B ligands derived from eliprodil. European Journal of Medicinal Chemistry, 237. https://doi.org/10.1016/j.ejmech.2022.114359

Masternak, M., Koch, A., Laulumaa, S., Tapken, D., Hollmann, M., Jørgensen, F. S., & Kastrup, J. S. (2022). Differences between the GluD1 and GluD2 receptors revealed by GluD1 X-ray crystallography, binding studies and molecular dynamics. FEBS Journal. https://doi.org/10.1111/febs.16631

Epping, L., Schroeter, C. B., Nelke, C., Bock, S., Gola, L., Ritter, N., Herrmann, A. M., Räuber, S., Henes, A., Wasser, B., Fernandez-Orth, J., Neuhaus, W., Bittner, S., Budde, T., Platten, M., Kovac, S., Seebohm, G., Ruck, T., Cerina, M., & Meuth, S. G. (2022). Activation of non-classical NMDA receptors by glycine impairs barrier function of brain endothelial cells. Cellular and Molecular Life Sciences, 79(9). https://doi.org/10.1007/s00018-022-04502-z.

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Product Manuals

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