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1 June
2022

Self-Paced Five-Choice Serial Reaction TimeTask for Assessment of Mouse Executive Function

1 June
2022

The authors present a self-paced protocol, suggesting quicker results may be obtained using mouse models.

Abstract

The five-choice serial reaction time-task (5-CSRTT) is a behavioral rodent test that can assess executive
functioning. It may be employed to analyze distinctive aspects of murine models for a plethora of diseases, such as attention deficit hyperactivity disorder, Alzheimer’s Disease, autism, and schizophrenia. The test has been performed primarily in rats and, although more challenging to apply to murine models, has the benefits of more diverse genetic models. The task is mainly based on training animals to nose-poke a lit screen within a stimulus duration time. Animals that perform a trial within the stimulus duration (SD) time receive a food/liquid reward. From training to experimental steps, classical 5-CSRTT can take more than 40 days to perform; however, here we present a murine self-paced, liquid reward-based 5-CSRTT (SP5C), using the ABET II system. Our version of SP5C can render competent results in ~14 days. We believe that, with this self-paced protocol, quicker results may be obtained using mouse models. Hence, a greater diversity of genetic models and diseases can be explored to expand our knowledge of complex polymorphic diseases, providing an additional platform for preclinical pharmaceutical testing.

Reference

Cunningham, J., Sheppard, L. D., Listik, E. and Wang, Q. (2022). Self-Paced Five-Choice Serial Reaction Time-Task for Mouse Behavioral Testing. Bio-101: e4388. DOI: 10.21769/BioProtoc.4388.

Our complete line of Rodent Touch Screen Chambers can be viewed here.

5 October
2021

Using touchscreen-delivered cognitive assessments to address the principles of the 3Rs

5 October
2021

A new article written by groups from University of Cambridge (UK), Western University (Ontario, Canada) and the Open University (UK) has reviewed the value of touchscreen use in addressing the principles of the 3Rs.

Abstract

Despite considerable advances in both in silico and in vitro approaches, in vivo studies that involve animal model systems remain necessary in many research disciplines. Neuroscience is one such area, with studies often requiring access to a complete nervous system capable of dynamically selecting between and then executing a full range of cognitive and behavioral outputs in response to a given stimulus or other manipulation. The involvement of animals in research studies is an issue of active public debate and concern and is therefore carefully regulated. Such regulations are based on the principles of the 3Rs of Replacement, Reduction and Refinement. In the sub-specialty of behavioral neuroscience, Full/Absolute Replacement remains a major challenge, as the complete ex vivo recapitulation of a system as complex and dynamic as the nervous system has yet to be achieved. However, a number of very positive developments have occurred in this area with respect to Relative Replacement and to both Refinement and Reduction. In this review, we discuss the Refinement- and Reduction-related benefits yielded by the introduction of touchscreen-based behavioral assessment apparatus. We also discuss how data generated by a specific panel of behavioral tasks developed for this platform might substantially enhance monitoring of laboratory animal welfare and provide robust, quantitative comparisons of husbandry techniques to define and ensure maintenance of best practice.

Reference

Lopez-Cruz, L., Bussey, T.J., Saksida, L.M., Heath, C.J. (2021) Using touchscreen-delivered cognitive assessments to address the principles of the 3Rs in behavioral sciencesLab Animal50, 174-184.

https://doi.org/10.1038/s41684-021-00791-2

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