Two Electrode Clamp for Large Cells

Two Electrode Clamp for Large Cells

Model npi.TEC-05X

Two-Electrode Voltage Clamp Amplifier for recordings from small & medium size cells

  • Reliable recordings of neuromuscular junctions or muscle cells
  • Stable recordings from invertebrate ganglion cells
  • Perform single electrode recordings in BRIDGE mode


  • Ideal amplifier for medium size cells such as invertebrate ganglion cells, muscle cells or neuromuscular junction preparations
  • Can be used with sharp microelectrodes, and patch pipettes in the whole-cell and perforated-patch configuration
  • Single electrode recording in BRIDGE mode: True current clamp operation with measured membrane potential and complete cancellation of series resistance (potential electrode)
  • Four-pole BESSEL filter for current
  • Digital DISPLAYS for current, voltage and electrode resistance
  • BUZZ and ELECTRODE CLEAR facility with remote hand or foot switch
  • OSCILLATION SHUT-OFF unit prevents cells from damage
  • From pA to µA – low voltage (±15 V) and high voltage (up to ±150 V) versions available
  • Easy connection to all major data acquisition systems, TTL control of MODE of OPERATION, telegraphing outputs for current sensitivity and filter



RPel: Potential Electrode Resistance Test;
CC: Current Clamp Mode;
VC: Voltage Clamp Mode;
RCel: Current Electrode Resistance Test;
BR: Bridge Mode (TEC-05X);
DHC Mode (TEC-05X, option);
Potential headstage:
Operating voltage ±15 V;
Size (approx.): 70x26x26mm, holding bar diameter 8 mm, length 150 mm;
Input resistance: >1013 Ω;
Differential input (for supression of bath potentials): cmr >80 dB.Current headstage (15 V, TEC-05X)):
Operating voltage: ±15 V;
Size (approx.): same as potential headstage,
Input resistance: >1012 Ω (internally trimmable).Current headstage (150 V):
Operating voltage: ±150 V (standard);
Size (approx.): 100x50x30 mm, grounded enclosure,
holding bar diameter 8 mm, length 10 cm (isolated from ground, standard system only);
Input resistance: >1012 Ω (internally trimmable);
Power dissipation: 6 W (standard)


Oscillation Shut-off
Electrode Resistance Test
Proportional Integrator
Series Resistance Compensation
Transient Compensation (TEC-10CX)


Uncompensated output signal: sensitivity 0.1 V/µA or 0.1 nA/V (TEC-05X), voltage range ±15 V;
Compensated/filtered output: sensitivity: 0.1 V…10 V/µA (V/nA for TEC-05X)
Potential electrode: two outputs, sensitivity x10 mV only (TEC-03X) or x10 mV and x40 mV (TEC-05X, TEC-10CX), voltage range ±15V;
Current electrode: sensitivity x10 mV, voltage range ±15 V;


CURRENT CLAMP (standard headstage):
Inputs: 1 µA/V, 0.1 µA/V with ON/OFF switches (TEC-10CX), 1 µA/V (TEC-03X), 1 nA/V, 0.1 nA/V with ON/OFF switches (TEC-05X);
input resistance >100 kΩ;
Inputs: :10 mV and :40 mV (TEC-05X, TEC-10CX) with ON/OFF switches, input resistance >100 kΩ;


MODE selection: rotary switches (TEC-03X, TEC-05X), or pushbuttons (TEC-10CX) , LED indicators;
remote selection by TTL inputs, software assessable (optional).
Current Electrode Parameter Controls:
Offset compensation: ten-turn control, ±1200 mV;
Capacity compensation (optional, TEC-05X): range 0 – 30 pF, ten-turn potentiometer.
Potential Electrode Parameter Controls:
Offset compensation: ±200mV, ten-turn control;
Capacity compensation: range 0 – 30 pF, ten-turn control.
Oscillation Shut off: disabled / off / reset switch, threshold set with linear control (0- 1200 mV).
Current Clamp: HOLD: X.XX µA (TEC-03X, TEC-10CX), X.XX nA, ten-turn digital control with -/0/+ switch, maximum 10 µA (TEC-03X, TEC-10CX) or 10 nA (TEC-05X);
BRIDGE balance (optional, TEC-05X systems): max. 10 MΩ or max. 100 MΩ (switch selected) with ten-turn digital control.
INTEGRATOR TIME CONSTANT: 200 µs – 2 ms, ten-turn control (TEC-03X) or control with ON/OFF switch (TEC-05X, TEC-10CX) ;
Voltage Clamp: HOLD: XXX mV, ten-turn digital control with +/0/- switch, maximum 1000 mV;
RISE TIME LIMIT: 0 – 0.2 ms;
GAIN: 10 µA/V – 10000 µA/V, ten-turn linear control;
INTEGRATOR TIME CONSTANT: 200 µs – 2 ms, ten-turn control (TEC-03X) or control with ON/OFF switch (TEC-05X, TEC-10CX) ;
OUTPUT CURRENT LIMIT5 (TEC-10CX): 0 – 100%, linear control;


Current output: lowpass  filters with 16 corner frequencies (20 Hz – 20 kHz)


Pitch correlated with potential signal.


potential display: XXX mV.
Electrode resistance display XXX MΩ.
current display: X.XX µA
VC error (TEC-10X)


115 V/230 V AC, 60 W (1.25 A/0.63 A fuse, SLOW).


19″ rackmount cabinet, 19″ (483 mm) wide, 14″ (355 mm) deep, 5.25″(132.5 mm) high
weight: approx. 8 kg.


Garifulina, A., Friesacher, T., Stadler, M., Zangerl-Plessl, E. M., Ernst, M., Stary-Weinzinger, A., Willam, A., & Hering, S. (2022). β subunits of GABAA receptors form proton-gated chloride channels: Insights into the molecular basis. Communications Biology, 5(1).

Lopez-Manzaneda, M., Fuentes-Moliz, A., & Tabares, L. (2022). Presynaptic Mitochondria Communicate With Release Sites for Spatio-Temporal Regulation of Exocytosis at the Motor Nerve Terminal. Frontiers in Synaptic Neuroscience, 14.

Mckenzie, C. E., Ho, C. J., Forster, I. C., Soh, M. S., Phillips, A. M., Chang, Y. C., Scheffer, I. E., Reid, C. A., & Tsai, M. H. (2022). Impaired Color Recognition in HCN1 Epilepsy: A Single Case Report. Frontiers in Neurology, 13.


Product Manuals

Related Products

Two Electrode Clamp for Oocytes
Two Electrode Clamp for Oocytes
Model npi.TEC-03X

The TEC-03X is based on the standard two electrode approach and is an ideal, easy-to-use system for recording from oocytes.

Copyright © 2024 Campden Instruments. All Rights Reserved.

Product Search

Use the provided form to search for products on this website.