Two Electrode Clamp for Large Cells
 

Two Electrode Clamp for Large Cells

Model npi.TEC-05X


Two-Electrode Voltage Clamp Amplifier for recordings from small & medium size cells

  • Reliable recordings of neuromuscular junctions or muscle cells
  • Stable recordings from invertebrate ganglion cells
  • Perform single electrode recordings in BRIDGE mode
 

Details

  • Ideal amplifier for medium size cells such as invertebrate ganglion cells, muscle cells or neuromuscular junction preparations
  • Can be used with sharp microelectrodes, and patch pipettes in the whole-cell and perforated-patch configuration
  • Single electrode recording in BRIDGE mode: True current clamp operation with measured membrane potential and complete cancellation of series resistance (potential electrode)
  • Four-pole BESSEL filter for current
  • Digital DISPLAYS for current, voltage and electrode resistance
  • BUZZ and ELECTRODE CLEAR facility with remote hand or foot switch
  • OSCILLATION SHUT-OFF unit prevents cells from damage
  • From pA to µA – low voltage (±15 V) and high voltage (up to ±150 V) versions available
  • Easy connection to all major data acquisition systems, TTL control of MODE of OPERATION, telegraphing outputs for current sensitivity and filter

Specifications

MODES OF OPERATION

RPel: Potential Electrode Resistance Test;
CC: Current Clamp Mode;
VC: Voltage Clamp Mode;
RCel: Current Electrode Resistance Test;
BR: Bridge Mode (TEC-05X);
DHC Mode (TEC-05X, option);
HEADSTAGES
Potential headstage:
Operating voltage ±15 V;
Size (approx.): 70x26x26mm, holding bar diameter 8 mm, length 150 mm;
Input resistance: >1013 Ω;
Differential input (for supression of bath potentials): cmr >80 dB.Current headstage (15 V, TEC-05X)):
Operating voltage: ±15 V;
Size (approx.): same as potential headstage,
Input resistance: >1012 Ω (internally trimmable).Current headstage (150 V):
Operating voltage: ±150 V (standard);
Size (approx.): 100x50x30 mm, grounded enclosure,
holding bar diameter 8 mm, length 10 cm (isolated from ground, standard system only);
Input resistance: >1012 Ω (internally trimmable);
Power dissipation: 6 W (standard)

FEATURES

Oscillation Shut-off
Electrode Resistance Test
Proportional Integrator
Series Resistance Compensation
Transient Compensation (TEC-10CX)

OUTPUTS

CURRENT OUTPUTS:
Uncompensated output signal: sensitivity 0.1 V/µA or 0.1 nA/V (TEC-05X), voltage range ±15 V;
Compensated/filtered output: sensitivity: 0.1 V…10 V/µA (V/nA for TEC-05X)
POTENTIAL OUTPUTS:
Potential electrode: two outputs, sensitivity x10 mV only (TEC-03X) or x10 mV and x40 mV (TEC-05X, TEC-10CX), voltage range ±15V;
Current electrode: sensitivity x10 mV, voltage range ±15 V;

INPUTS

CURRENT CLAMP (standard headstage):
Inputs: 1 µA/V, 0.1 µA/V with ON/OFF switches (TEC-10CX), 1 µA/V (TEC-03X), 1 nA/V, 0.1 nA/V with ON/OFF switches (TEC-05X);
input resistance >100 kΩ;
VOLTAGE CLAMP:
Inputs: :10 mV and :40 mV (TEC-05X, TEC-10CX) with ON/OFF switches, input resistance >100 kΩ;

CONTROLS

MODE selection: rotary switches (TEC-03X, TEC-05X), or pushbuttons (TEC-10CX) , LED indicators;
remote selection by TTL inputs, software assessable (optional).
Current Electrode Parameter Controls:
Offset compensation: ten-turn control, ±1200 mV;
Capacity compensation (optional, TEC-05X): range 0 – 30 pF, ten-turn potentiometer.
Potential Electrode Parameter Controls:
Offset compensation: ±200mV, ten-turn control;
Capacity compensation: range 0 – 30 pF, ten-turn control.
Oscillation Shut off: disabled / off / reset switch, threshold set with linear control (0- 1200 mV).
Current Clamp: HOLD: X.XX µA (TEC-03X, TEC-10CX), X.XX nA, ten-turn digital control with -/0/+ switch, maximum 10 µA (TEC-03X, TEC-10CX) or 10 nA (TEC-05X);
BRIDGE balance (optional, TEC-05X systems): max. 10 MΩ or max. 100 MΩ (switch selected) with ten-turn digital control.
INTEGRATOR TIME CONSTANT: 200 µs – 2 ms, ten-turn control (TEC-03X) or control with ON/OFF switch (TEC-05X, TEC-10CX) ;
Voltage Clamp: HOLD: XXX mV, ten-turn digital control with +/0/- switch, maximum 1000 mV;
RISE TIME LIMIT: 0 – 0.2 ms;
GAIN: 10 µA/V – 10000 µA/V, ten-turn linear control;
INTEGRATOR TIME CONSTANT: 200 µs – 2 ms, ten-turn control (TEC-03X) or control with ON/OFF switch (TEC-05X, TEC-10CX) ;
OUTPUT CURRENT LIMIT5 (TEC-10CX): 0 – 100%, linear control;

OUTPUT FILTERS

Current output: lowpass  filters with 16 corner frequencies (20 Hz – 20 kHz)

AUDIO MONITOR

Pitch correlated with potential signal.

DISPLAYS

potential display: XXX mV.
Electrode resistance display XXX MΩ.
current display: X.XX µA
VC error (TEC-10X)

POWER REQUIREMENTS

115 V/230 V AC, 60 W (1.25 A/0.63 A fuse, SLOW).

DIMENSIONS

19″ rackmount cabinet, 19″ (483 mm) wide, 14″ (355 mm) deep, 5.25″(132.5 mm) high
weight: approx. 8 kg.

Science

Garifulina, A., Friesacher, T., Stadler, M., Zangerl-Plessl, E. M., Ernst, M., Stary-Weinzinger, A., Willam, A., & Hering, S. (2022). β subunits of GABAA receptors form proton-gated chloride channels: Insights into the molecular basis. Communications Biology, 5(1). https://doi.org/10.1038/s42003-022-03720-2

Lopez-Manzaneda, M., Fuentes-Moliz, A., & Tabares, L. (2022). Presynaptic Mitochondria Communicate With Release Sites for Spatio-Temporal Regulation of Exocytosis at the Motor Nerve Terminal. Frontiers in Synaptic Neuroscience, 14. https://doi.org/10.3389/fnsyn.2022.858340

Mckenzie, C. E., Ho, C. J., Forster, I. C., Soh, M. S., Phillips, A. M., Chang, Y. C., Scheffer, I. E., Reid, C. A., & Tsai, M. H. (2022). Impaired Color Recognition in HCN1 Epilepsy: A Single Case Report. Frontiers in Neurology, 13. https://doi.org/10.3389/fneur.2022.834252

Media

Product Manuals

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