Two-Electrode Voltage Clamp Amplifier for routine recordings from oocytes
| MODES OF OPERATION | RPel: Potential Electrode Resistance Test; CC: Current Clamp Mode; VC: Voltage Clamp Mode; RCel: Current Electrode Resistance Test; BR: Bridge Mode (TEC-05X); DHC Mode (TEC-05X, option); HEADSTAGES Potential headstage: Operating voltage ±15 V; Size (approx.): 70x26x26mm, holding bar diameter 8 mm, length 150 mm; Input resistance: >1013 Ω; Differential input (for supression of bath potentials): cmr >80 dB.Current headstage (15 V, TEC-05X)): Operating voltage: ±15 V; Size (approx.): same as potential headstage, Input resistance: >1012 Ω (internally trimmable).Current headstage (150 V): Operating voltage: ±150 V (standard); Size (approx.): 100x50x30 mm, grounded enclosure, holding bar diameter 8 mm, length 10 cm (isolated from ground, standard system only); Input resistance: >1012 Ω (internally trimmable); Power dissipation: 6 W (standard) |
| FEATURES | Oscillation Shut-off Electrode Resistance Test Proportional Integrator Series Resistance Compensation Transient Compensation (TEC-10CX) |
| OUTPUTS | CURRENT OUTPUTS: Uncompensated output signal: sensitivity 0.1 V/µA or 0.1 nA/V (TEC-05X), voltage range ±15 V; Compensated/filtered output: sensitivity: 0.1 V…10 V/µA (V/nA for TEC-05X) POTENTIAL OUTPUTS: Potential electrode: two outputs, sensitivity x10 mV only (TEC-03X) or x10 mV and x40 mV (TEC-05X, TEC-10CX), voltage range ±15V; Current electrode: sensitivity x10 mV, voltage range ±15 V; |
| INPUTS | CURRENT CLAMP (standard headstage): Inputs: 1 µA/V, 0.1 µA/V with ON/OFF switches (TEC-10CX), 1 µA/V (TEC-03X), 1 nA/V, 0.1 nA/V with ON/OFF switches (TEC-05X); input resistance >100 kΩ; VOLTAGE CLAMP: Inputs: :10 mV and :40 mV (TEC-05X, TEC-10CX) with ON/OFF switches, input resistance >100 kΩ; |
| CONTROLS | MODE selection: rotary switches (TEC-03X, TEC-05X), or pushbuttons (TEC-10CX) , LED indicators; remote selection by TTL inputs, software assessable (optional). Current Electrode Parameter Controls: Offset compensation: ten-turn control, ±1200 mV; Capacity compensation (optional, TEC-05X): range 0 – 30 pF, ten-turn potentiometer. Potential Electrode Parameter Controls: Offset compensation: ±200mV, ten-turn control; Capacity compensation: range 0 – 30 pF, ten-turn control. Oscillation Shut off: disabled / off / reset switch, threshold set with linear control (0- 1200 mV). Current Clamp: HOLD: X.XX µA (TEC-03X, TEC-10CX), X.XX nA, ten-turn digital control with -/0/+ switch, maximum 10 µA (TEC-03X, TEC-10CX) or 10 nA (TEC-05X); BRIDGE balance (optional, TEC-05X systems): max. 10 MΩ or max. 100 MΩ (switch selected) with ten-turn digital control. INTEGRATOR TIME CONSTANT: 200 µs – 2 ms, ten-turn control (TEC-03X) or control with ON/OFF switch (TEC-05X, TEC-10CX) ; Voltage Clamp: HOLD: XXX mV, ten-turn digital control with +/0/- switch, maximum 1000 mV; RISE TIME LIMIT: 0 – 0.2 ms; GAIN: 10 µA/V – 10000 µA/V, ten-turn linear control; INTEGRATOR TIME CONSTANT: 200 µs – 2 ms, ten-turn control (TEC-03X) or control with ON/OFF switch (TEC-05X, TEC-10CX) ; OUTPUT CURRENT LIMIT5 (TEC-10CX): 0 – 100%, linear control; |
| OUTPUT FILTERS | Current output: lowpass filters with 16 corner frequencies (20 Hz – 20 kHz) |
| AUDIO MONITOR | Pitch correlated with potential signal. |
| DISPLAYS | potential display: XXX mV. Electrode resistance display XXX MΩ. current display: X.XX µA VC error (TEC-10X) |
| POWER REQUIREMENTS | 115 V/230 V AC, 60 W (1.25 A/0.63 A fuse, SLOW). |
| DIMENSIONS | 19″ rackmount cabinet, 19″ (483 mm) wide, 14″ (355 mm) deep, 5.25″(132.5 mm) high weight: approx. 8 kg. |
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