Vibratomes for Botanical Research: Accurate, Efficient, and Versatile Plant Sectioning

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Vibratomes for Botanical Research: Accurate, Efficient, and Versatile Plant Sectioning

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Introduction

In the world of botany, precision and accuracy are essential for studying plant structures at a microscopic level. Plant samples vary greatly in texture, density, and composition, requiring a vibratome that offers adjustable parameters to ensure optimal sectioning. Our vibrating microtomes are designed to provide thin, high-quality slices with minimal damage while considering how samples vary to ensure that researchers can slice plant tissues with ease with customizable slicing parameters. Whether you are working in academia, agriculture, or biotechnology, our vibratomes offer the reliability you need to answer your research questions.

Read the Application Note:

Key Features of Campden Vibratomes for Plant Sectioning:

  1. Customizable and Automated Slicing Parameters 
    • Different plant samples require varied slicing conditions for optimal results. 
    • The 7000smz-2 offers a wide range of settings: 
    • Vibration Frequency: 50-120 Hz (most vibratomes only offer 85 Hz, which is not ideal for all tissues) 
    • Blade Vibration Amplitude: 0.5-2.5 mm. 
    • Advance Speed: 0.01 – 1 mm/sec, with a 4 mm/sec quick retraction to optimize slicing time. 
    • Users can modify slicing parameters in real-time, and the vibratome remembers these changes preventing errors and saving time. This is useful when slicing plant samples embedded in agar—faster slicing through agar, then slowing down for the plant itself, then speeding up again at the end. 
  2. Exceptional Durability & Cost Efficiency 
    • The Campden vibratome is highly reliable, requiring minimal servicing. 
    • No service contract is needed, and routine maintenance is only recommended after 500 hours or 10 years. 
    • Even after 14 years on the market, most units still function without servicing, saving customers money. 
  3. High-Quality Blades 
    • Custom-designed ceramic and stainless steel blades prevent tissue compression to ensure clean, undamaged plant tissue sections. 
    • Standard razor blades are blunt and uneven, making them unsuitable for precision slicing. 
  4. Easy to Use 
    • Designed for both beginners and experts in precision sectioning enabling experiments to begin immediately upon plugging in the vibratome. Our start guide can be found here. 

 

 

7000smz-2 Vibratome
7000smz-2 Vibratome

Model 7000smz-2

Our top of the range high precision, vibrating microtome, this is the finest tissue slicer in the world for preparations for visual patch clamping. Shown with optional Slice incubation chamber and temperature controller.

5100mz-Plus Vibratome
5100mz-Plus Vibratome

Model 5100mz-Plus

The 5100-Plus is perfect for those who need to keep slices viable for longer e.g. for electrophysiological field recordings. The user can calibrate the Z-axis deflection of the blade to 2 µm with the adjustable blade holder and "Opti-cal" Calibration device.

5100mz Vibratome
5100mz Vibratome

Model 5100mz

The 5100mz is a very competitively priced, high precision, vibrating microtome (vibrotome for short) which shares many features with the top of the range 7000smz series, such as the vibrating mechanism, the inner and outer tissue baths and the easy to use control system.

Conclusion

With the user-friendly design, our vibratomes enable researchers to unlock new insights in the plant sciences. Whether your sample has or has not been sliced before, we encourage you to try slicing on a Campden vibratome.

To learn more about what plant scientists around the world have done using Campden vibratomes, check out the application note above and explore references from users across the globe to see how our vibratomes are being used. 

Selected References

2024

Pan, H., Shim, A., Lubin, M. B., & Belin, B. J. (2024). Hopanoid lipids promote soybean–Bradyrhizobium symbiosis. Mbio, 15(4), e02478-23. https://doi.org/10.1128/mbio.02478-23 

2023

Hermanowicz, P., Giza, A., & Łabuz, J. (2023). Sensitive detection of chloroplast movements through changes in leaf cross-polarized reflectance. bioRxiv, 2023-10. https://doi.org/10.1101/2023.10.24.563792  

2022 

Coles, D. W., Bithell, S. L., Mikhael, M., Cuddy, W. S., & Plett, J. M. (2022). Chickpea roots undergoing colonisation by Phytophthora medicaginis exhibit opposing jasmonic acid and salicylic acid accumulation and signalling profiles to leaf hemibiotrophic models. Microorganisms, 10(2), 343.  https://doi.org/10.3390/microorganisms10020343 

Fusi, R., Rosignoli, S., Lou, H., Sangiorgi, G., Bovina, R., Pattem, J. K., ... & Salvi, S. (2022). Root angle is controlled by EGT1 in cereal crops employing an antigravitropic mechanism. Proceedings of the National Academy of Sciences, 119(31), e2201350119. https://doi.org/10.1073/pnas.220135011

Kumar, P., Cowan, G. H., Squires, J. N., Hackett, C. A., Tobin, A. K., Torrance, L., & Roberts, A. G. (2022). Phloem connectivity and transport are not involved in mature plant resistance (MPR) to Potato Virus Y in different potato cultivars, and MPR does not protect tubers from recombinant strains of the virus. Journal of Plant Physiology, 275, 153729. https://doi.org/10.1016/j.jplph.2022.153729 

Plett, K. L., Snijders, F., Castañeda‐Gómez, L., Wong‐Bajracharya, J. W. H., Anderson, I. C., Carrillo, Y., & Plett, J. M. (2022). Nitrogen fertilization differentially affects the symbiotic capacity of two co‐occurring ectomycorrhizal species. Environmental Microbiology, 24(1), 309-323. https://doi.org/10.1111/1462-2920.15879 

Vanhees, D. J., Schneider, H. M., Sidhu, J. S., Loades, K. W., Bengough, A. G., Bennett, M. J., ... & Lynch, J. P. (2022). Soil penetration by maize roots is negatively related to ethylene‐induced thickening. Plant, Cell & Environment, 45(3), 789-804. https://doi.org/10.1111/pce.14175 

2021 

Ballesteros, H. G. F., Rosman, A. C., Carvalho, T. L. G., Grativol, C., & Hemerly, A. S. (2021). Cell wall formation pathways are differentially regulated in sugarcane contrasting genotypes associated with endophytic diazotrophic bacteria. Planta, 254, 1-21. https://doi.org/10.1007/s00425-021-03768-0  

De Meester, B., Vanholme, R., de Vries, L., Wouters, M., Van Doorsselaere, J., & Boerjan, W. (2021). Vessel‐and ray‐specific monolignol biosynthesis as an approach to engineer fiber‐hypolignification and enhanced saccharification in poplar. The Plant Journal, 108(3), 752-765. https://doi.org/10.1111/tpj.15468 

Jones, D. H., Atkinson, B. S., Ware, A., Sturrock, C. J., Bishopp, A., & Wells, D. M. (2021). Preparation, scanning and analysis of duckweed using x-ray computed microtomography. Frontiers in Plant Science, 11, 617830. https://doi.org/10.3389/fpls.2020.617830  

2020 and Prior 

Burr, C. A., Sun, J., Yamburenko, M. V., Willoughby, A., Hodgens, C., Boeshore, S. L., ... & Kieber, J. J. (2020). The HK5 and HK6 cytokinin receptors mediate diverse developmental pathways in rice. Development, 147(20), dev191734. https://doi.org/10.1242/dev.19173

Plett, J. M., Plett, K. L., Wong‐Bajracharya, J., de Freitas Pereira, M., Costa, M. D., Kohler, A., ... & Anderson, I. C. (2020). Mycorrhizal effector PaMiSSP10b alters polyamine biosynthesis in Eucalyptus root cells and promotes root colonization. New Phytologist, 228(2), 712-727.  https://doi.org/10.1111/nph.16759 

De Meester, B., De Vries, L., Özparpucu, M., Gierlinger, N., Corneillie, S., Pallidis, A., ... & Boerjan, W. (2018). Vessel-specific reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in dwarfed ccr1 mutants restores vessel and xylary fiber integrity and increases biomass. Plant Physiology, 176(1), 611-633. https://doi.org/10.1104/pp.17.01462 

Yu, S., Huang, A., Li, J., Gao, L., Feng, Y., Pemberton, E., & Chen, C. (2018). OsNAC45 plays complex roles by mediating POD activity and the expression of development-related genes under various abiotic stresses in rice root. Plant Growth Regulation, 84, 519-531. https://doi.org/10.1007/s10725-017-0358-0  

Atkinson, J. A., & Wells, D. M. (2017). An updated protocol for high throughput plant tissue sectioning. Frontiers in plant science, 8, 1721. https://doi.org/10.3389/fpls.2017.01721 

 

 

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