BA-03X-Bridge Amplifier
 

BA-03X-Bridge Amplifier

Model npi.BA-03X


  • True current clamp operation with direct measurement of membrane potential and complete cancellation of series resistance and stray capacitance
  • Can be used with sharp microelectrodes, or patch pipettes in whole-cell, cell-attached and perforated-patch configuration
  • Can be used for extracellular recordings of sub millivolt signals as well
  • POTENTIAL OUTPUT GAIN: 10, 20, 50, 100, 200, 500, 1k
  • POTENTIAL HIGHPASS filter: DC: 0.1, 0.3, 0.5, 1, 3, 10, 30, 50, 100, 200, 500, 600, 1k, 3k Hz
  • POTENTIAL LOWPASS filter: 20, 50, 100, 200, 300, 500, 700, 1k, 1.3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k Hz
  • CURRENT OUTPUT SENSITIVITY: 0.1, 0.2, 0.5, 1, 2, 5, 10V / nA
  • Digital DISPLAYS for current, voltage and electrode resistance
  • BUZZ and ELECTRODE CLEAR facility, AUDIO monitor
  • OSCILLATION SHUT-OFF unit prevents cells from damage
  • TTL gates stimulus input with amplitude setting by 3-digit potentiometer and digital read-out of holding current
  • Enhanced (x10) current range for Juxtracellular recordings, electroporation or Iontophoresis
 

Details

The BA amplifiers are wide-bandwidth high impedance amplifier designed for intracellular current clamp recordings with sharp microelectrodes, in perforated or tight seal patch configurations as well as for electroporation and iontophoresis. Currently, npi BA amplifiers are used in vertebrate (rat, mouse, cat, fish etc.) and invertebrate (leech, crab, snail, insect etc.) neurons, muscle cells, other excitable tissue and plant cells.

Each amplifier of the BA series is housed in a 19″ rackmount cabinet and equipped with functions like two ranges for bridge balance, an oscillation shut-off circuit for protecting the preparation, two potential and two current ranges. The amplifier comes with a small headstage with a holding bar (or a mounting plate / dovetail adapter on request) and a BNC connector for the electrode holder, and can be attached directly to a micromanipulator.

The BA-01X is the standard bridge amplifier fot intracellular current clamp recordings. Having an extended current range (x10, i.e. +/. 120 nA max.) this amplifer is also suitable for electroporation of single cells for non-invasive, juxtacellular filling of cells with dyes or plasmids.

The BA-03X has additionally high and low pass filters for the recorded potenial, and a potential gain up to 1000. Therefore, it allows extracellular recordings as well.

A bridge amplifier is also available as module for the EPMS-07 system.

Specifications

 

HEADSTAGE Input voltage range: ±1000 mV
Operating voltage: ±15 V
Enclosure: Size: 23 x 70 x 26 mm, grounded
Holding bar: Size: length 150 mm, diameter 8 mm
Electrode connector: BNC connector with driven shield
Ground connector: 2.4 mm connector
Input resistance: >1013 Ω
Current range x1: ±12 nA into 100 MΩ
Current range x10: ±120 nA into 100 MΩ
ELECTRODE PARAMETER CONTROLS BIAS: ±150 pA in range x1 and x10, adjustable with ten-turn control
OFFSET: ten-turn control, range ±200 mV
CAPACITY COMPENSATION: range 0-30 pF, ten-turn control.
CELL PENETRATION Overcompensation of capacity compensation (BUZZ), duration ~2 – ~50 ms (slightly dependent on the setting of capacitance compensation), timer controlled with linear control,
accessible with remote switch
ELECTRODE CLEAR CIRCUIT Application of max. DC currents (-Imax or +Imax),
OSCILLATION SHUT-OFF Turns off current injection and capacity compensation, function indicated by red / green LED,
disabled / off / reset switch, threshold set with linear control (0-1200 mV).
BRIDGE BALANCE adjustable with ten-turn control, 0-100 MΩ and 0-1000 MΩ with toggle switch.
ELECTRODE RESISTANCE test 10 mV / MΩ obtained by application of square current pulses ±1 nA, display XXXX MΩ.
BANDWIDTH and SPEED of RESPONSE Full power bandwidth (Re = 0): >30 kHz, rise time (10 % – 90 %) <10 µs (Re = 100 MΩ),
<5 µs (Re = 5 MΩ).
OUTPUTS (BA-01X) Impedance: 50 Ω;
Current x1: sensitivity 0.1 V / nA, display XX.XX nA, resolution 10 pA;
Current x10: sensitivity 0.1V / nA, display XXX.X nA, resolution 100 pA;
Potential: BNC connector, sensitivity x10 mV or x100 mV, selectable with toggle switch, display XXXX mV.
OUTPUTS (BA-03X) Impedance: 50 Ω;
Current range x1: sensitivity 1 V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XX.XX nA, resolution 10 pA;
Current range x10: sensitivity 0.1V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XXX.X nA, resolution 100 pA;
Potential: BNC connector, sensitivity x10 mV to x1000 mV, selectable with rotary switch (7 steps), display XXXX mV.
CURRENT INPUTS RANGE x1 0.1 nA / V, 1 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 10 pA, polarity selectable with toggle switch,
holding current range ±10 nA with ten-turn control.
CURRENT INPUTS RANGE x10 1 nA / V, 10 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 100 pA, polarity selectable with toggle switch,
holding current range ±100 nA with ten-turn control.
POWER REQUIREMENTS 115 / 230 V AC, 25 W
DIMENSIONS 19″ rackmount cabinet, 19″ (483 mm) wide, 10″ (250 mm) deep, 3.5″ (88 mm) high
HEADSTAGE Input voltage range: ±1000 mV
Operating voltage: ±15 V
Enclosure: Size: 23 x 70 x 26 mm, grounded
Holding bar: Size: length 150 mm, diameter 8 mm
Electrode connector: BNC connector with driven shield
Ground connector: 2.4 mm connector
Input resistance: >1013 Ω
Current range x1: ±12 nA into 100 MΩ
Current range x10: ±120 nA into 100 MΩ
ELECTRODE PARAMETER CONTROLS BIAS: ±150 pA in range x1 and x10, adjustable with ten-turn control
OFFSET: ten-turn control, range ±200 mV
CAPACITY COMPENSATION: range 0-30 pF, ten-turn control.
CELL PENETRATION Overcompensation of capacity compensation (BUZZ), duration ~2 – ~50 ms (slightly dependent on the setting of capacitance compensation), timer controlled with linear control,
accessible with remote switch
ELECTRODE CLEAR CIRCUIT Application of max. DC currents (-Imax or +Imax),
OSCILLATION SHUT-OFF Turns off current injection and capacity compensation, function indicated by red / green LED,
disabled / off / reset switch, threshold set with linear control (0-1200 mV).
BRIDGE BALANCE adjustable with ten-turn control, 0-100 MΩ and 0-1000 MΩ with toggle switch.
ELECTRODE RESISTANCE test 10 mV / MΩ obtained by application of square current pulses ±1 nA, display XXXX MΩ.
BANDWIDTH and SPEED of RESPONSE Full power bandwidth (Re = 0): >30 kHz, rise time (10 % – 90 %) <10 µs (Re = 100 MΩ),
<5 µs (Re = 5 MΩ).
OUTPUTS (BA-01X) Impedance: 50 Ω;
Current x1: sensitivity 0.1 V / nA, display XX.XX nA, resolution 10 pA;
Current x10: sensitivity 0.1V / nA, display XXX.X nA, resolution 100 pA;
Potential: BNC connector, sensitivity x10 mV or x100 mV, selectable with toggle switch, display XXXX mV.
OUTPUTS (BA-03X) Impedance: 50 Ω;
Current range x1: sensitivity 1 V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XX.XX nA, resolution 10 pA;
Current range x10: sensitivity 0.1V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XXX.X nA, resolution 100 pA;
Potential: BNC connector, sensitivity x10 mV to x1000 mV, selectable with rotary switch (7 steps), display XXXX mV.
CURRENT INPUTS RANGE x1 0.1 nA / V, 1 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 10 pA, polarity selectable with toggle switch,
holding current range ±10 nA with ten-turn control.
CURRENT INPUTS RANGE x10 1 nA / V, 10 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 100 pA, polarity selectable with toggle switch,
holding current range ±100 nA with ten-turn control.
POWER REQUIREMENTS 115 / 230 V AC, 25 W
DIMENSIONS 19″ rackmount cabinet, 19″ (483 mm) wide, 10″ (250 mm) deep, 3.5″ (88 mm) high

Science

Han, M., Zhao, J., Fabian, J. M., Evans, S., Mustafa, S., Ruan, Y., Wiederman, S., & Ebendorff-Heidepriem, H. (2021). Cytoplasmic delivery of quantum dots via microelectrophoresis technique. Electrophoresis, 42(11), 1247–1254. https://doi.org/10.1002/ELPS.202000388

Nguyen, T. A. T., Beetz, M. J., Merlin, C., & El Jundi, B. (2021). Sun compass neurons are tuned to migratory orientation in monarch butterflies. Proceedings of the Royal Society B: Biological Sciences, 288(1945). https://doi.org/10.1098/rspb.2020.2988

Bothe, M. S., Luksch, H., Straka, H., & Kohl, T. (2019). Neuronal Substrates for Infrared Contrast Enhancement and Motion Detection in Rattlesnakes. Current Biology, 29(11), 1827-1832.e4. https://doi.org/10.1016/j.cub.2019.04.035

Kepple, D., Cazakoff, B. N., Demmer, H. S., Eckmeier, D., Shea, S. D., & Koulakov, A. A. (2018). Computational algorithms and neural circuitry for compressed sensing in the mammalian main olfactory bulb. BioRxiv. https://doi.org/10.1101/339689

Xu, W., & Baker, S. N. (2018). In vitro characterization of intrinsic properties and local synaptic inputs to pyramidal neurons in macaque primary motor cortex. European Journal of Neuroscience, 48(4), 2071–2083. https://doi.org/10.1111/ejn.14076

Scherberich, J., Hummel, J., Schöneich, S., & Nowotny, M. (2017). Functional basis of the sexual dimorphism in the auditory fovea of the duetting bushcricket Ancylecha fenestrata. Proceedings of the Royal Society B: Biological Sciences, 284(1865). https://doi.org/10.1098/rspb.2017.1426

Du, C., Collins, W., Cantley, W., Sood, D., & Kaplan, D. L. (2017). Tutorials for Electrophysiological Recordings in Neuronal Tissue Engineering. ACS Biomaterials Science and Engineering, 3(10), 2235–2246. https://doi.org/10.1021/acsbiomaterials.7b00318

 

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