4 Channel PlexBright Optogenetic Controller with Radiant Software

Details

The PlexBright 4 Channel Optogenetic Controller controlled by Radiant Software is one of the cornerstones of the PlexBright Optogenetic Stimulation System (PlexBright System). The newest version of Radiant, version 2.0, was released at the start of 2014 and offers unsurpassed control of LEDs or lasers over multiple channels.

Through Radiant’s easy-to-use GUI, each 4 Channel Optogenetic Controller starts, drives and stops up to four channels independently from the most basic signals to the generation of sophisticated output patterns. Further, one channel may drive an LED module, while a second channel drives a laser.

Each channel/output may be configured to deliver up to 1100mA of precision-controlled current to one or two PlexBright LED Modules. Alternatively, outputs may be configured to deliver up to a 5V signal, enabling simultaneous control of both LED modules and lasers. The 4 Channel Optogenetic Controller further enables interaction with third party equipment through eight digital inputs and 16 digital outputs.

Radiant 2.0’s pattern generation functionality allows researchers to quickly define simple to complex output patterns that may be saved and played back repeatedly. Arbitrary stimulation patterns can also be loaded from user defined text files, or the outputs can be controlled directly by the user in manual mode. Patterns can also be generated in an offline mode for use at a later time.

With respect to LEDs – and like the PlexBright LD-1 Single Channel LED Driver – each output of the 4 Channel Optogenetic Controller is capable of controlling one or two LED modules. When two modules share a single output simultaneously (accomplished via a specialized cable that connects the LED Modules in series called a PlexBright Series-Y BNC cable), each module receives the same control signal. This configuration is particularly applicable to bilateral stimulation experiments.

For higher volume research experiments, Radiant enables the integration of up to four PlexBright 4 Channel Optogenetic Controllers simultaneously operated by a single computer – yielding up to 16 channels of independent output.

PlexBright Starter Kits offer the initial products to get started and a 10% discount when purchased as a kit. Kits are available in four experimental designs and with either driver – the 4 Channel Optogenetic Controller with Radiant Software (fully functional kit) or our other device, the LD-1 Single Channel LED Driver (economical kit).

PlexBright Quote Request Forms (also specific to the experimental set-up) include the Starter Kit itemization and individual parts for selection. The forms are available on the Resources tab, or on the Documentation page under the PlexBright header.

In addition to the PlexBright 4 Channel Optogenetic Controller, the PlexBright System also offers the PlexBright LD-1 Single Channel LED Driver; a full range of super intensity PlexBright LED Modules; several PlexBright compatible commutator options; high performance PlexBright Optical Patch Cables with a choice of stimulation tips; specially designed PlexBright Fiber Stub Implants; the PlexBright Light Measurement Kit; and the PlexBright Optical Fiber Cleaning Kit to safely maintain and extend the life of the fiber tips.

Specifications

Feature	Specifications and Options	Remarks
PlexBright Starter Kits	Freely Behaving Mouse & Small Animal Kit Freely Behaving Rat & Large Animal Kit Head-fixed/Anesthetized Animal Kit In vitro Experiment Kit	Starter Kits offer the initial set-up products and a 10% discount when purchased as a kit. Kits are available in four experimental design set-ups and with either driver (the 4 Channel Controller with Radiant Software or the LD-1 Single-Channel LED Driver).
Hardware	PlexBright 4 Channel Optogenetic Controller
Light source compatibility	PlexBright LED Modules and Third-party lasers	LED compatibility includes all PlexBright LED Modules. Laser compatibility includes those equipped with a controller that accepts a 0-5V analog modulation input.
Analog output channels	Up to 4	Each channel offers independently controllable output.
Channel synchronization	Capable	All four channels may be started at the same time.
Stimulation modes	Current Voltage	Each channel is independently controlled – including the simulation mode. Thus, it is possible to perform LED stimulation on one channel and a laser on another simultaneously.
Current output	Up to 1100mA	Current output for driving PlexBright LED Modules.
Voltage output	0 to 5V	Voltage output for controlling a third-party laser.
Temporal resolution	100ìs
Digital inputs	8	Two per channel. May be used to start and stop stimulation on individual channels.
Digital outputs	16	Four per channel.
Minimum trigger pulse width	200µs
Arbitrary waveform update rate	10kHz
Commutator compatibility	PlexBright Dual LED Commutator PlexBright Dual LED + 16 Channel Commutator	If the identical parameters are desired for both modules on the commutator – such as for bilateral stimulation – a PlexBright Series-Y BNC Cable will also be required.
Dimensions	4.8 x 9.7 x 20.3cm 1.9 x 3.8 x 8.0 inches	Includes feet, switches, and BNC connectors in length and height.
Weight	0.5kg 1.2lbs
4 Channel Optogenetic Controller includes	Optogenetic stimulator Power supply and power cord USB cable Four color-coded insulated BNC cables (1 per channel)
Software	Radiant™	Radiant is the user interface and signal visualization program operating the PlexBright 4 Channel Controller. It supports multiple devices simultaneously.
Pattern generation	Capable	Radiant is capable of generating complex signal patterns independently per channel.
LED module compatibility	All PlexBright LED Modules	PlexBright LED Module identification within Radiant automatically sets current limits.
Number of LED modules controlled per channel simultaneously	1 or 2	Outputs can operate a single PlexBright LED Module or two for bilateral stimulation in which identical parameters are desired for both stimulation sites. A PlexBright Series-Y BNC Cable is required for operating two modules from a single channel.
Devices per Radiant license	Up to 4	Radiant enables up to four PlexBright 4 Channel Controllers to be operated simultaneously on a single computer per license – enabling up to 16 total channels.
Operating Systems	Windows® XP Windows 7
Activation requirement	Plexon version-specific hardware license key	Radiant v2 requires a license key for operation. One license key is provided with the purchase of the controller and software.
Accessories	PlexBright Series-Y BNC Cable	The PlexBright Series-Y BNC Cable enables the simultaneous operation of two PlexBright LED Modules from a single channel in which identical parameters are desired for both modules.

Science

Lukasz, C., Stanislawa, J. J., Kamil, P., Daniela, K. J., Monika, B., Michal, W., Mariusz, K., & Henryk, L. M. (2020). Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat. The Journal of Physiology. https://doi.org/10.1113/jp280275

Wilson, C. A., Fouda, S., & Sakata, S. (2020). Effects of optogenetic stimulation of basal forebrain parvalbumin neurons on Alzheimer’s disease pathology. Scientific Reports, 10(1), 2020.04.26.062950. https://doi.org/10.1038/s41598-020-72421-9

Nieves, G. M., Bravo, M., & Bath, K. (2020). Early life adversity decreases fear expression in pre-adolescence by accelerating amygdalar parvalbumin cell development. BioRxiv, 2020.01.07.892687. https://doi.org/10.1101/2020.01.07.892687

Chen, J. Y., Fu, E. J., Patel, P. R., Hostetler, A. J., Sawan, H. A., Moss, K. A., Hocevar, S. E., Anderson, A. J., Chestek, C. A., & Shea, L. D. (2020). Lentiviral Interleukin-10 Gene Therapy Preserves Fine Motor Circuitry and Function After a Cervical Spinal Cord Injury in Male and Female Mice. Neurotherapeutics. https://doi.org/10.1007/s13311-020-00946-y

Ansorge, J., Humanes‐Valera, D., Pauzin, F. P., Schwarz, M. K., & Krieger, P. (2020). Cortical layer 6 control of sensory responses in higher‐order thalamus. The Journal of Physiology, 598(18), 3973–4001. https://doi.org/10.1113/JP279915

Rossier, D., Franca, V. La, Salemi, T., & Gross, C. T. (2020). A neural circuit for competing approach and avoidance underlying prey capture. BioRxiv. https://doi.org/10.1101/2020.08.24.265181

Murphy, A. J., Hasse, J. M., & Briggs, F. (2020). Physiological characterization of a rare subpopulation of doublet-spiking neurons in the ferret lateral geniculate nucleus. Journal of Neurophysiology, 124(2), 432–442. https://doi.org/10.1152/jn.00191.2020

Wang, L., & Krauzlis, R. J. (2020). Involvement of Striatal Direct Pathway in Visual Spatial Attention in Mice. Current Biology. https://doi.org/10.1016/j.cub.2020.08.083

Nieves, G. M., Bravo, M., & Bath, K. (2020). Early life adversity decreases fear expression in pre-adolescence by accelerating amygdalar parvalbumin cell development. Preprint. https://doi.org/10.1101/2020.01.07.892687

Ito, W., Fusco, B., & Morozov, A. (2020). Disinhibition-assisted long-term potentiation in the prefrontal-amygdala pathway via suppression of somatostatin-expressing interneurons. Neurophotonics, 7(01), 1. https://doi.org/10.1117/1.nph.7.1.015007

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